Presynaptic NMDA Receptors Influence Ca2+ Dynamics by Interacting with Voltage-Dependent Calcium Channels during the Induction of Long-Term Depression.

Spike-timing-dependent long-term depression (t-LTD) of glutamatergic layer (L)4-L2/3 synapses in developing neocortex requires activation of astrocytes by endocannabinoids (eCBs), which release glutamate onto presynaptic NMDA receptors (preNMDARs). The exact function of preNMDARs in this context is still elusive and strongly debated. To elucidate their function, we show that bath application of the eCB 2-arachidonylglycerol (2-AG) induces a preNMDAR-dependent form of chemically induced LTD (eCB-LTD) in L2/3 pyramidal neurons in the juvenile somatosensory cortex of rats. Presynaptic Ca2+ imaging from L4 spiny stellate axons revealed that action potential (AP) evoked Ca2+ transients show a preNMDAR-dependent broadening during eCB-LTD induction. However, blockade of voltage-dependent Ca2+ channels (VDCCs) did not uncover direct preNMDAR-mediated Ca2+ transients in the axon. This suggests that astrocyte-mediated glutamate release onto preNMDARs does not result in a direct Ca2+ influx, but that it instead leads to an indirect interaction with presynaptic VDCCs, boosting axonal Ca2+ influx. These results reveal one of the main remaining missing pieces in the signaling cascade of t-LTD at developing cortical synapses

Strategic and operational decision-making in expanding supply chains for LNG as a fuel

The European Union aims for a 40% reduction in greenhouse gas emissions by 2030, compared to 1990 levels, and recognizes the opportunities of Liquefied Natural Gas (LNG) as an alternative fuel for transportation to reach this goal. The lack of a mature supply chain for LNG as a fuel results in a need to invest in new (satellite) terminals, bunker barges and tanker trucks. This network design problem can be defined as a Two-Echelon Capacitated Location Routing Problem with Split Deliveries (2E-CLRPSP). An important feature of this problem is that direct deliveries are allowed from terminals, which makes the problem much harder to solve than the existing location routing literature suggests. In this paper, we improve the performance of a hybrid exact algorithm and apply our algorithm to a real world network design problem related to the expansion of the European supply chain for LNG as a fuel. We show that satellite terminals and bunker barges become an interesting option when demand for LNG grows and occurs further away from the import terminal. In those situations, the large investments associated with LNG satellites and bunker barges are offset by reductions in operational costs of the LNG tanker trucks

Are JWST/NIRCam color gradients in the lensed z=2.3 dusty star-forming galaxy El Anzuelo due to central dust attenuation or inside-out galaxy growth?

Gradients in the mass-to-light ratio of distant galaxies impede our ability to characterize their size and compactness. The long-wavelength filters of $JWST$'s NIRCam offer a significant step forward. For galaxies at Cosmic Noon ($z\sim2$), this regime corresponds to the rest-frame near-infrared, which is less biased towards young stars and captures emission from the bulk of a galaxy's stellar population. We present an initial analysis of an extraordinary lensed dusty star-forming galaxy (DSFG) at $z=2.3$ behind the $El~Gordo$ cluster ($z=0.87$), named $El~Anzuelo$ ("The Fishhook") after its partial Einstein-ring morphology. The FUV-NIR SED suggests an intrinsic star formation rate of $81^{+7}_{-2}~M_\odot~{\rm yr}^{-1}$ and dust attenuation $A_V\approx 1.6$, in line with other DSFGs on the star-forming main sequence. We develop a parametric lens model to reconstruct the source-plane structure of dust imaged by the Atacama Large Millimeter/submillimeter Array, far-UV to optical light from $Hubble$, and near-IR imaging with 8 filters of $JWST$/NIRCam, as part of the Prime Extragalactic Areas for Reionization and Lensing Science (PEARLS) program. The source-plane half-light radius is remarkably consistent from $\sim 1-4.5~\mu$m, despite a clear color gradient where the inferred galaxy center is redder than the outskirts. We interpret this to be the result of both a radially-decreasing gradient in attenuation and substantial spatial offsets between UV- and IR-emitting components. A spatial decomposition of the SED reveals modestly suppressed star formation in the inner kiloparsec, which suggests that we are witnessing the early stages of inside-out quenching.Comment: 29 pages, 11 figures, 5 tables. Accepted for publication in Ap

The JWST Discovery of the Triply-imaged Type Ia "Supernova H0pe" and Observations of the Galaxy Cluster PLCK G165.7+67.0

A Type Ia supernova (SN) at $z=1.78$ was discovered in James Webb Space Telescope Near Infrared Camera imaging of the galaxy cluster PLCK G165.7+67.0 (G165; $z = 0.35$). The SN is situated 1.5-2kpc from its host galaxy Arc 2 and appears in three different locations as a result of gravitational lensing by G165. These data can yield a value for Hubble's constant using time delays from this multiply-imaged SN Ia that we call "SN H0pe." Over the entire field we identified 21 image multiplicities, confirmed five of them using Near-Infrared Spectrograph (NIRspec), and constructed a new lens model that gives a total mass within 600kpc of ($2.6 \pm 0.3) \times 10^{14}$ M$_{\odot}$. The photometry uncovered a galaxy overdensity at Arc 2's redshift. NIRSpec confirmed six member galaxies, four of which surround Arc 2 with relative velocity $\lesssim$900 km s$^{-1}$ and projected physical extent $\lesssim$33 kpc. Arc 2 dominates the stellar mass ($(5.0 \pm 0.1) \times 10^{11}$ M$_{\odot}$), which is a factor of ten higher than other members of this compact galaxy group. These other group members have specific star formation rates (sSFR) of 2-260Gyr$^{-1}$ derived from the H$\alpha$-line flux corrected for stellar absorption, dust extinction, and slit losses. Another group centered on the dusty star forming galaxy Arc 1 is at $z=2.24$. The total SFR for the Arc 1 group ($gtrsim$ M$_{\odot}$ yr$^{-1}$) translates to a supernova rate of $\sim$1 SNe yr$^{-1}$, suggesting that regular monitoring of this cluster may yield additional SNe.Comment: 27 pages, submitted to Ap

Hidden giants in JWST's PEARLS: An ultra-massive z=4.26 sub-millimeter galaxy that is invisible to HST

We present a multi-wavelength analysis using SMA, JCMT, NOEMA, JWST, HST, and SST of two dusty strongly star-forming galaxies, 850.1 and 850.2, seen through the massive cluster lens A1489. These SMA-located sources both lie at z=4.26 and have bright dust continuum emission, but 850.2 is a UV-detected Lyman-break galaxy, while 850.1 is undetected at <2um, even with deep JWST/NIRCam observations. We investigate their stellar, ISM, and dynamical properties, including a pixel-level SED analysis to derive sub-kpc-resolution stellar-mass and Av maps. We find that 850.1 is one of the most massive and highly obscured, Av~5, galaxies known at z>4 with M*~10^11.8 Mo (likely forming at z>6), and 850.2 is one of the least massive and least obscured, Av~1, members of the z>4 dusty star-forming population. The diversity of these two dust-mass-selected galaxies illustrates the incompleteness of galaxy surveys at z>3-4 based on imaging at <2um, the longest wavelengths feasible from HST or the ground. The resolved mass map of 850.1 shows a compact stellar mass distribution, Re(mass)~1kpc, but its expected evolution to z~1.5 and then z~0 matches both the properties of massive, quiescent galaxies at z~1.5 and ultra-massive early-type galaxies at z~0. We suggest that 850.1 is the central galaxy of a group in which 850.2 is a satellite that will likely merge in the near future. The stellar morphology of 850.1 shows arms and a linear bar feature which we link to the active dynamical environment it resides within.Comment: Submitted to ApJ, comments welcome

Hidden Giants in JWST's PEARLS: An Ultramassive z = 4.26 Submillimeter Galaxy that Is Invisible to HST

We present a multiwavelength analysis using the Submillimeter Array (SMA), James Clerk Maxwell Telescope, NOEMA, JWST, the Hubble Space Telescope (HST), and the Spitzer Space Telescope of two dusty strongly star-forming galaxies, 850.1 and 850.2, seen through the massive cluster lens A 1489. These SMA-located sources both lie at z = 4.26 and have bright dust continuum emission, but 850.2 is a UV-detected Lyman-break galaxy, while 850.1 is undetected at ≲ 2 μm, even with deep JWST/NIRCam observations. We investigate their stellar, interstellar medium, and dynamical properties, including a pixel-level spectral energy distribution analysis to derive subkiloparsec-resolution stellar-mass and A V maps. We find that 850.1 is one of the most massive and highly obscured, A V ∼ 5, galaxies known at z > 4 with M * ∼1011.8 M ⊙ (likely forming at z > 6), and 850.2 is one of the least massive and least obscured, A V ∼ 1, members of the z > 4 dusty star-forming population. The diversity of these two dust-mass-selected galaxies illustrates the incompleteness of galaxy surveys at z ≳ 3–4 based on imaging at ≲ 2 μm, the longest wavelengths feasible from HST or the ground. The resolved mass map of 850.1 shows a compact stellar-mass distribution, Remass ∼1 kpc, but its expected evolution means that it matches both the properties of massive, quiescent galaxies at z ∼ 1.5 and ultramassive early-type galaxies at z ∼ 0. We suggest that 850.1 is the central galaxy of a group in which 850.2 is a satellite that will likely merge in the near future. The stellar morphology of 850.1 shows arms and a linear bar feature that we link to the active dynamical environment it resides within

Paper 1: The JWST PEARLS View of the El Gordo Galaxy Cluster and of the Structure It Magnifies

The massive galaxy cluster El Gordo (z=0.87) imprints multitudes of gravitationally lensed arcs onto James Webb Space Telescope (JWST) Near-Infrared Camera (NIRCam) images. Eight bands of NIRCam imaging were obtained in the ``Prime Extragalactic Areas for Reionization and Lensing Science'' (``PEARLS'') program. PSF-matched photometry across Hubble Space Telescope (HST) and NIRCam filters supplies new photometric redshifts. A new light-traces-mass lens model based on 56 image multiplicities identifies the two mass peaks and yields a mass estimate within 500 kpc of ~(7.0 +/- 0.30) x 10^14 Msun. A search for substructure in the 140 cluster members with spectroscopic redshifts confirms the two main mass components. The southeastern mass peak that contains the BCG is more tightly bound than the northwestern one. The virial mass within 1.7 Mpc is (5.1 +/- 0.60) x 10^14 Msun, lower than the lensing mass. A significant transverse velocity component could mean the virial mass is underestimated. We contribute one new member to the previously known z=4.32 galaxy group. Intrinsic (delensed) positions of the five secure group members span a physical extent of ~60 kpc. Thirteen additional candidates selected by spectroscopic/photometric constraints are small and faint with a mean intrinsic luminosity ~2.2 mag fainter than L*. NIRCam imaging admits a fairly wide range of brightnesses and morphologies for the group members, suggesting a more diverse galaxy population in this galaxy overdensity.Comment: 24 pages, accepted by Ap

Two HIV-1 Variants Resistant to Small Molecule CCR5 Inhibitors Differ in How They Use CCR5 for Entry

HIV-1 variants resistant to small molecule CCR5 inhibitors recognize the inhibitor-CCR5 complex, while also interacting with free CCR5. The most common genetic route to resistance involves sequence changes in the gp120 V3 region, a pathway followed when the primary isolate CC1/85 was cultured with the AD101 inhibitor in vitro, creating the CC101.19 resistant variant. However, the D1/86.16 escape mutant contains no V3 changes but has three substitutions in the gp41 fusion peptide. By using CCR5 point-mutants and gp120-targeting agents, we have investigated how infectious clonal viruses derived from the parental and both resistant isolates interact with CCR5. We conclude that the V3 sequence changes in CC101.19 cl.7 create a virus with an increased dependency on interactions with the CCR5 N-terminus. Elements of the CCR5 binding site associated with the V3 region and the CD4-induced (CD4i) epitope cluster in the gp120 bridging sheet are more exposed on the native Env complex of CC101.19 cl.7, which is sensitive to neutralization via these epitopes. However, D1/86.16 cl.23 does not have an increased dependency on the CCR5 N-terminus, and its CCR5 binding site has not become more exposed. How this virus interacts with the inhibitor-CCR5 complex remains to be understood

JWST PEARLS. Prime Extragalactic Areas for Reionization and Lensing Science: Project Overview and First Results

We give an overview and describe the rationale, methods, and first results from NIRCam images of the JWST “Prime Extragalactic Areas for Reionization and Lensing Science” (PEARLS) project. PEARLS uses up to eight NIRCam filters to survey several prime extragalactic survey areas: two fields at the North Ecliptic Pole (NEP); seven gravitationally lensing clusters; two high redshift protoclusters; and the iconic backlit VV 191 galaxy system to map its dust attenuation. PEARLS also includes NIRISS spectra for one of the NEP fields and NIRSpec spectra of two high-redshift quasars. The main goal of PEARLS is to study the epoch of galaxy assembly, active galactic nucleus (AGN) growth, and First Light. Five fields—the JWST NEP Time-Domain Field (TDF), IRAC Dark Field, and three lensing clusters—will be observed in up to four epochs over a year. The cadence and sensitivity of the imaging data are ideally suited to find faint variable objects such as weak AGN, high-redshift supernovae, and cluster caustic transits. Both NEP fields have sightlines through our Galaxy, providing significant numbers of very faint brown dwarfs whose proper motions can be studied. Observations from the first spoke in the NEP TDF are public. This paper presents our first PEARLS observations, their NIRCam data reduction and analysis, our first object catalogs, the 0.9–4.5 μm galaxy counts and Integrated Galaxy Light. We assess the JWST sky brightness in 13 NIRCam filters, yielding our first constraints to diffuse light at 0.9–4.5 μm. PEARLS is designed to be of lasting benefit to the community